A new method to make proteins without the use of live cells
Anti aging, Body Building, Buy hgh, Guide, HgH, News, Review
Now the medicine and the HGH industry has reasons to smile because Cornell biological engineers have developed a new method which offers an efficient way to make proteins for use in medicine or HGH industry without the use of live cells.
Current methods employ vats of genetically modified bacteria or mammalian cells that churn out proteins for such pharmaceuticals as insulin or human growth hormone (hgh). But there are many proteins that bacteria or cells cannot tolerate and would kill the host if overproduced inside a cell. With this new method many such proteins can be made which cannot be produced by current biotechnology.
Researchers have tried mixing DNA that codes for the desired protein with the amino acids from which proteins are made along with ribosomes (cell structures that assemble proteins) and other helper chemicals in a test tube. Cornell’s faster, more efficient process weaves the coding DNA into an artificial gel made of synthetic DNA.
Pads of P-gel, each about 1 millimeter square, in which DNA has been embedded to code for a fluorescent compound.
Luo’s group has pioneered the use of synthetic DNA as a self-assembling construction material. Strands of DNA that are designed to be complementary over a small part of their length can join together into various shapes. In this application they form crosses, which in turn link at their ends to form a 3-D matrix. This makes a hydrogel, a spongy material that absorbs water without dissolving in the water.
To make a protein-producing gel, which Luo calls a P-gel, the synthetic DNA is also made to include sequences that join to the ends of plasmids — strands of DNA that code for the desired protein. A mix of X-shaped and plasmid DNA then assembles into a gel with genes coding for the desired protein integrated throughout. To increase the surface area for reaction, tiny drops of the P-gel are molded into pads about 1 millimeter square by 20 microns (millionths of a meter) thick. Several hundred pads are then placed in a solution of amino acids and protein-making machinery extracted from living cells.
The result, Luo reports, is to produce proteins up to 300 times more efficiently than when the same reactions are carried out with DNA floating freely in the same solution. The system has so far been tested with 16 proteins, including several that are toxic or would otherwise be impossible to make in living cells.
(Source: http://www.physorg.com/)
Popularity: 2% [?]
 |
   |